To dress the question if other virulence gene were present in this kind of strains , 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study . the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e . coli ( etec ) , invasive plasmid antigen b ( ipab ) for enteroinvasive e . coli ( eiec ) , epec adherence factor ( eaf ) , epec secretion protein c ( espc ) for enteropathogenic e . coli ( epec ) , hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e . coli ( ehec ) and eaggec probe for entero - aggregative e . coli ( eaggec ) . the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e . coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected , the later was a newly discovered putative pathogenicity island in e . coli o157 : h7 為探討攜帶小腸結(jié)腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因,選取82株由原位雜交和pcr方法初篩irp2陽(yáng)性的大腸桿菌菌株,進(jìn)行在致瀉性大腸桿菌的25個(gè)毒力基因的檢測(cè),包括腸產(chǎn)毒性大腸桿菌的熱穩(wěn)定毒素st和熱不穩(wěn)定毒素lt ,腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ,腸致病性大腸桿菌的eaf 、 espc基因,腸出血性大腸桿菌的溶血素hly 、志賀毒素1 ( slt1 ) 、志賀毒素2 ( slt2 )基因,腸集聚性大腸桿菌的eaggec探針,以及在泌尿道致病性大腸桿菌和o157 : h7大腸桿菌中新發(fā)現(xiàn)的毒力島基因。